info.improove@gmail.com
+91 81788 23424

Experiment 28: Action of Enzymes on starch

Experiment 28: Action of Enzymes on starch

Objective

To study the action of salivary amylase on starch solution

Materials required:

  • 1% starch solution
  • 1% iodine solution
  • saliva
  • a measuring cylinder
  • test tubes
  • a dropper
  • a spatula (or spoon)

Procedure

  • Collect some saliva from the mouth cavity in a test tube. Add 10 mL water to it. Label this test tube as saliva solution (S)
  • Take 2 mL of 1% starch solution in two different test tubes. Label the test tubes as A and B
  • Add 1 mL diluted saliva from S to B. Shake thoroughly. Do not add anything to A
  • After about five minutes using a dropper, take five drops of solution from A and add two drops of 1% iodine solution. Mix the solution by stirring. Observe the colour of the mixture and record your observation
  • Take five drops from B. Add two drops of 1% iodine solution to it. Record your observation
  • Repeat steps 4 and 5, after five, ten, fifteen and twenty minutes

Observations and Findings:

  • It takes less time to reach achromic point at 37°C, as the enzyme is maximum active at this temperature, while at higher and lower temperatures more time is taken to reach the achromic point.

Conclusions:

  • Over time, salivary amylase breaks starch and converts it into sugar. We observe that starch solution turns iodine blue in the beginning but no change in color happens later, suggesting that starch has been converted to sugar

Theory:

  • Saliva is one of the digestive juices secreted by salivary glands present in the mouth cavity. It contains an enzyme called salivary amylase (ptyalin) that acts on starch and converts it into simpler sugars.

Experiment-based learning kits

img
Chemistry Lab in A Box (Upto Class 10)

Comprehensive chemistry kit for Class 6 to 10

  • All reagents and lab equipment needed to do over 33 experiments as per Board syllabus

  • High quality and safe lab reagents

  • Videos of all the experiments Detailed instruction booklet with theory, concepts and procedures

MRPRs 4,499/-
AmazonRs 2,349/-
Buy on Amazon
FlipkartRs 2,399/-
Buy on Flipkart
HereRs 1,999/-
Buy Now
img
Refill for home experiment kits

Set of chemicals and reagents for home experiments

  • Support for all major science activity kits like Kutuhal, Einstein Box, Genius Minds, etc.

  • High quality and safe lab reagents

  • Strict quality testing procedures to ensure safety while doing experiments

MRPRs 2,499/-
AmazonRs 1,149/-
Buy on Amazon
FlipkartRs 1,199/-
Buy on Flipkart
HereRs 999/-
Buy Now!!
img
Basic chemistry equipment (starter kit)

Basic equipment to generate excitement towards science

  • Basic set of equipment like test tubes, holder, cleaner, filter papers, pipettes, litmus papers, etc.

  • High quality and safe lab equipment

  • Good starting point for children (suitable for children aged 7-10 years)

MRPRs 1,499/-
AmazonRs 499/-
Buy on Amazon
FlipkartRs 499/-
Buy on Flipkart
HereRs 449/-
Buy Now!!
img
Crystallography @ home

Make bright-colored crystals of different shapes and sizes

  • All reagents and equipment needed to make bright crystals - from multiple compounds

  • Instruction booklet and videos

  • Learn about crystallography - crystal shapes, solubility, effect of temperature, etc.

MRPRs 1,499/-
AmazonRs 1,149/-
Buy on Amazon
FlipkartRs 1,149/-
Buy on Flipkart
HereRs 999/-
Buy Now!!